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AAT Bioquest

FAQ

Posted on March 9, 2023
What are the procedures of preparation of the biological specimens to be analyzed under a microscope?
FixationMicroscopyParaffin embedded sectionsfixed cells


Posted on March 9, 2023
What is the procedure for heat fixation?
FixationIHCImmunohistochemistry (IHC)Microscopybacteriafixed cells


Posted on December 8, 2022
What are the factors that will affect the rate and effectiveness of tissue fixation?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What are the types of fixatives?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What are the general types of fixation?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What are the commonly used chemical fixatives for microscopy?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What are the functions of fixative?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What are the properties of an ideal fixative?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What are the differences between formalin and formaldehyde?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
Is formaldehyde a crosslinking agent?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
Why do tissues require fixation?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What is the purpose of cell fixation?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on August 3, 2021
Do you have any dyes that can stain the nuclei in live cells? Are they also fixable and can I use them to stain fixed cells?
Nuclei StainingDNAFluorescenceemission wavelengthfixablefixed cellsfluorescence imaginglive cell analysisnuclear DNAMicroscopy


Posted on April 17, 2020
Which is the best conditions for methanol fixation of microtubules?
Bioconjugation Chemical ReagentsClassic DyesFixationPhysiological Probesfixed cells


Posted on April 17, 2020
What is the difference between PLL and PDL for surface coating?
Cell CultureFixationadherent cellsfixed cells Chemical Reagentsfluorescence microscope


Posted on April 17, 2020
How can I attach suspension cells to glass slides for fluorescence microscopy investigation?
FixationPBSfixed cellsfluorescence microscope Chemical Reagents


Posted on April 17, 2020
Techniques for storage of tissues post-fixation?
FixationStoragefixed cells Chemical ReagentsImmunohistochemistry (IHC)


Posted on April 17, 2020
What is the best fixation for fluorescence microscopy of GFP tagged proteins?
FixationPBSfixed cellsfluorescence microscope


Posted on January 4, 2020
How does phalloidin affect actin assembly?
iFluor dyesActinCell CytotoxicityF-actinPhalloidinactin polymerization inhibitoractin stainingcell-impermeablefluorescently labeled phalloidinphalloidin conjugatesfixed cells


Posted on October 24, 2019
Is phalloidin cell permeable?
actin stainingactinF-actinphalloidinphalloidin conjugatesfixed cellscell-impermeable